Scalable Stirred-Suspension Bioreactor Culture of Human Pluripotent Stem Cells
نویسندگان
چکیده
منابع مشابه
Controlling Expansion and Cardiomyogenic Differentiation of Human Pluripotent Stem Cells in Scalable Suspension Culture
To harness the potential of human pluripotent stem cells (hPSCs), an abundant supply of their progenies is required. Here, hPSC expansion as matrix-independent aggregates in suspension culture was combined with cardiomyogenic differentiation using chemical Wnt pathway modulators. A multiwell screen was scaled up to stirred Erlenmeyer flasks and subsequently to tank bioreactors, applying control...
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The promise of human pluripotent stem cell (hPSC) therapies is ever-increasing, with the rapid proliferation of reports over the past several years demonstrating homogeneous and efficient differentiation of hPSCs to clinically relevant cell types (1–3). Such discoveries have renewed the enthusiasm and motivation for the acceleration of clinical trials using hPSC-derived cells to treat nervous s...
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The replacement of regenerated thyroid follicular cells (TFCs) is a promising therapeutic strategy for patients with hypothyroidism. Here, we have succeeded in inducing functional TFCs from human-induced pluripotent stem cells (iPSCs) in scalable suspension culture. Differentiation of iPSCs with Activin A treatment produced Sox17- and FoxA2-expressing definitive endodermal cells that also expre...
متن کاملAggregate formation and suspension culture of human pluripotent stem cells and differentiated progeny.
Culture of human pluripotent stem cells (hPSC) as in vitro multicellular aggregates has been increasingly used as a method to model early embryonic development. Three-dimensional assemblies of hPSCs facilitate interactions between cells and their microenvironment to promote morphogenesis, analogous to the multicellular organization that accompanies embryogenesis. In this paper, we describe a me...
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Current laboratory methods used to passage adherent human pluripotent stem cells (hPSCs) are labor intensive, result in reduced cell viability and are incompatible with larger scale production necessary for many clinical applications. To meet the current demand for hPSCs, we have developed a new non-enzymatic passaging method using sodium citrate. Sodium citrate, formulated as a hypertonic solu...
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ژورنال
عنوان ژورنال: Tissue Engineering Part A
سال: 2010
ISSN: 1937-3341,1937-335X
DOI: 10.1089/ten.tea.2009.0454